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I started giving my 9 year old golden retriever cosequin recently for stiffness. She had tibial-plateau-leveling osteotomy (TPLO) surgery when she was 3.

To get her to eat it, I grind up the pills and mix it in with a small amount of yogurt or tuna twice a day.

Would it impact the effectiveness to mix the morning and night batches at the same time and leave one in the fridge all day?

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I would advise against it. I am not familiar with the typical bacteria living and growing in a can of tuna after opening it, but with the yogurt, the bacteria in there will metabolize the glucosamine. This will definitively impact the effectiveness. I strongly suspect this would at least to some extent also happen with the tuna.

Here is one example of Lactobacillus bacteria able to ferment glucosamine. I did not add this before because using glucosamine as a nutrient is not uncommon in general for bacteria (like it is not for humans or dogs).

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  • Do you have any references supporting your claim that yogurt cultures will metabolize the glucosamine? – James Jenkins Aug 17 '17 at 10:17
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    "bacteria in yogurt" are not necessarily just the yogurt cultures, but anything that likes lactic acid. Metabolizing glucosamine is not uncommon in those, as far as I remember. (In fact more like that common that it is not specifically published anymore.) – skymningen Aug 17 '17 at 15:06

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