A few months ago my girlfriend and 2 cats moved in, and my dog (9 year old labrador) got shut out from the bedroom. Due to the large attached bath, it was the most logical place for their litterbox and therefore safe space for them. My dog never slept in the bedroom with me anyway, but he used to have access. Now he's shut out with a baby gate. He started out barking at night wanting to come in, but that was easy to ignore until it stopped.
Now, after I came back from a week long trip (so possible separation issues? My girlfriend stayed home with him and said he was fine while I was gone), he has started barking in the morning, ranging from 2am to 6am, but mostly around 4:30-5am. I thought this would end on its own, but it's carried on for 6 weeks now.
The problem is that it's hard to ignore. It wakes us up, but then once he sees me move he doesn't carry on, which makes it hard to "not respond to it" or do any kind of irritation such as squirt bottle or noise. The sonic collar I got doesn't detect his bark, only his head scratching and my bark.